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KMID : 1129720210380030140
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Korean Journal of Acupuncture
2021 Volume.38 No. 3 p.140 ~ p.150
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Psoraleae Semen Ethanol Extract Inhibits RANKL-Induced Osteoclast Differentiation and Osteoclast Specific Genes Expression
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Ryu Gwang-Hyun
Kim Eom-Ji
Kim Min-Sun
Kim Jae-Hyun
Lee Yu-Jin
Jin Dae-Hwan
Sohn Young-Joo
Jung Hyuk-Sang
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Abstract
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Objectives : The increase of osteoclasts could cause osteoporosis and bone-related diseases. Also, the inhibition of osteoclast differentiation is important in treating bone-related diseases. Traditionally, Psoraleae Semen has been used for geriatric diseases, aging and musculoskeletal diseases. The purpose of this study is to investigate the effect of Psoraleae Semen ethanol extract (PS) on osteoclast differentiation and its function.
Methods : To confirm the effect of PS on osteoclastogenesis and bone resorption activity, various levels of concentrations of PS (5, 10, 20 and 40 μg/ml) were tested on RAW 264.7 cells cultured with RANKL. We measured tartarate-resistant acid phosphatase (TRAP)-positive cells, TRAP activity, pit formation and F-actin ring formation. The expressions of nuclear factor of activated T-cells (NFATc1) and c-Fos were confirmed through western blot and reverse transcription- polymerase chain reaction (RT-PCR). Also, the expression of bone resorption and fusion-related genes in osteoclast was confirmed by RT-PCR.
Results : PS decreased the number of TRAP-positive cells and the TRAP activity. In addition, PS significantly inhibited the formation of pit and F-actin ring. Furthermore, PS decreased the expression of osteoclast related genes.
Conclusions : PS inhibits osteoclast differentiation and bone resorption ability through inhibition of the expression of osteoclastrelated genes. This indicates that PS may be a potential therapeutic agent to osteoporosis by suppressing osteoclastogenesis.
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KEYWORD
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Psoraleae Semen, osteoclast, RANKL, NFATc1, c-Fos
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